Introgression of Brassica napus genome into restorer lines for CMS ogura estimated with the use of molecular markers

• Authors: W. Poplawska , I. Bartkowiak-Broda
• Languages of publication: PL

Abstracts

EN

The object of research were double low restorer lines of CMS ogura. The restorer gene was introgressed into rapeseed genotype from radish (Raphanus sativus) through intergeneric hybridisation. The obtained recombinants of rapeseed with the restorer gene comprise a DNA fragment originating from radish, which is bigger than the locus of the restorer gene. It disturbs the first meiotic behaviour in PMC. In addition, it has an impact on the fertility and yielding ability of restorer lines. Moreover, the restorer gene in the obtained recombinant is tightly linked with a gene responsible for high content of undesirabled compounds in seeds - glucosinolates. Elimination of the unnecessary DNA fragment is performed by backcrosses with double low lines of winter rapeseed. The changes in rapeseed genome which are a result of backcrosses are investigated by the use of molecular and isozyme markers. Key words: Brassica napus, restorer gene, introgression, molecular and isozyme markers. Adres do korespondencji:

Keywords

EN

BRASSICA NAPUS   INTROGRESSION   MARKER   RESTORER GENE  

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2001
• Issue: 1
• Pages: 112-118
• Document Type: ARTICLE
• Publication order reference: W. Poplawska, Instytut Hodowli i Aklimatyzacji Roslin, Zaklad Roslin Oleistych, ul. Strzeszynska 36, 60-479 Poznan, Poland, e-mail: ibart @ nico.ihar.poznan. pl