New polymorphism of FASN gene in chicken

• Authors: G. Marrube , F. Rozen , G.B. Pinto , N. Pacienza , J.E. Melo , M.J. Huguet , Z. Canet , R. Zandomeni , M.C. Miquel
• Languages of publication: EN

Abstracts

EN

Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.

Discipline

• ANIMAL_SCIENCES: ANIMAL SCIENCES

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2004
• Volume: 45
• Issue: 4
• Pages: 453-455

Contributors

author

G. Marrube

author

F. Rozen

author

G.B. Pinto

author

N. Pacienza

author

J.E. Melo

author

M.J. Huguet

author

Z. Canet

author

R. Zandomeni

author

M.C. Miquel

• Document Type: SHORT COMMUNICA
• Publication order reference: G. Marrube, Genetics Area, Animal Production Department, Veterinary School, University of Buenos Aires, Chorroarin 280, 1427, Buenos Aires, Argentina