Effectiveness of Agrobacterium-mediated transformation in selected forms of tomato (Lycopersicon esculentum Mill.) and preliminary characteristics of transgenic plants.
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Three forms of Lycopersicon esculentum Mill. variety Beta, line ls and line nor were subject to Agrobacterium-mediated transformation with two constructs: pBI121 and pRUR528. In both cases transgenic plants were obtained which formed roots in a medium with kanamycin and showed expected band in the PCR analysis. The effectiveness of the transformation, measured as the number of explants forming structures on selection medium, was different for the used genotypes when a bacterial strain carrying plasmid pRUR528 was used. No genotype-dependent difference in effectiveness of the transformation was observed in the case of pBI121. The genotype-dependent differences in the effectiveness of the transgenic shoots regeneration and rooting were observed. 76% of transgenic plants showed unchanged ploidy level.
Publication order reference
G.Bartoszewski, Katedra Genetyki, Hodowli i Biotechnologii Roslin, Wydzial Ogrodniczy SGGW, ul. Nowoursynowska 166; 02-787 Warszawa, Poland