Genetically engineered potato resistant to potato leafroll virus infection
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Binary plasmids carrying different fragments of the coat protein gene of potato leafroll luteovirus were constructed for transformation of commercial potato cultivar Bzura. Several transgenic plant lines were obtained and characterised. The resistance of transgenic plants to the virus was tested by inoculation with aphids or grafting. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. Expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants.
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D.Hulanicka, Zaklad Biochemii Drobnoustrojow, Instytut Biochemii i Biofizyki PAN, ul. Pawinskiego 5a, 02-106 Warszawa, Poland, fax: 022 658 46 36.