Rosa indica 'Major' is a vegetatively propagated rootstock, valuable for greenhouse grown cultivars. In vitro propagation could help the supply of high quality plants for field stock plantations. Results from our experiments show that increase in the number of shoots >1 cm can be obtained through the use of 2,2 g/l Gelrite instead of Plant agar. Reduction of 6-benzylaminopurine concentration to 1,5 mg/l. FeEDDHA chelate did not improve the quality of the cultures. Rooting of shoots could be more effective when riboflavine is added to the auxin medium in a one-step procedure or when microcuttings are subcultured after root induction to auxin free medium.