Erythropoietin preconditioning suppresses neuronal death following status epilepticus in rats

• Authors: J. Yang , Y. Huang , X. Yu , H. Sun , Y. Li , Y. Deng
• Languages of publication: EN

Abstracts

EN

Status epilepticus (SE) is a grave condition in which the brain undergoes lasting seizures which can lead to neuronal loss. Our previous study suggested that preconditioning with erythropoietin (Epo) suppressed neuronal apoptosis in hippocampus of rats following SE in vivo by inhibiting caspase-3. In this study, we investigated the mechanisms by which Epo preconditioning may exert its anti-apoptotic effects using a lithium-pilocarpine induced SE model in rats. The effects of Epo on neuronal cell death were evaluated using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and the role of the Bcl-2 protein family, which have been shown to be anti- (Bcl-2, Bcl-w) or pro- (Bid, Bim) apoptotic, was examined with immunofluorescence. We found Epo preconditioning decreased the total number of TUNEL, Bim and Bid positive cells, but increased the total number of Bcl-w and Bcl-2 positive cells. These results suggest that systemic Epo pretreatment protects neurons in an acute phase of SE and may result in further suppression of neuronal apoptosis in hippocampus by regulating the balance between pro- and anti-apoptotic Bcl-2 family proteins.

Keywords

EN

APOPTOSIS; EPILEPSY; HIPPOCAMPUS; RAT

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2007
• Volume: 67
• Issue: 2
• Pages: 141-148

Contributors

author

J. Yang

author

Y. Huang

author

X. Yu

author

H. Sun

author

Y. Li

author

Y. Deng

• Document Type: ARTICLE
• Publication order reference: Y. Huang, Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China