Isolation and chromosomal distribution of a novel Ty1-copia-like sequence from Secale, which enables identification of wheat?Secale africanum introgression lines

• Authors: J. Jia , Z. Yang , G. Li , Ch. Liu , M. Lei , T. Zhang , J. Zhou , Z. Ren
• Languages of publication: EN

Abstracts

EN

A repetitive sequence of 411 bp, named pSaO5411, was identified in the Secale africanum genome (Ra) by random amplified polymorphic DNA (RAPD) analysis of wheat and wheat?S. africanum amphiploids. GenBank BLAST search revealed that the sequence of pSaO5411 was highly homologous to a part of a Ty1-copia retrotransposon. Fluorescence in situ hybridization (FISH) analyses indicated that pSaO5411 was significantly hybridized to S. africanum chromosomes of a wheat?S. africanum amphiploid, and it was dispersed along the Secale chromosome arms except the terminal regions. Basing on the sequence of pSaO5411, a pair of sequence-characterized amplified region (SCAR) primers were designed, and the resultant SCAR marker was able to target both cultivated rye and the wild Secale species, which also enabled to identify effectively the S. africanum chromatin introduced into the wheat genome.

Keywords

EN

FISH; MOLECULAR MARKER; RETROTRANSPOSON; SECALE AFRICANUM

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2009
• Volume: 50
• Issue: 1
• Pages: 25-28

Contributors

author

J. Jia

author

Z. Yang

author

G. Li

author

Ch. Liu

author

M. Lei

author

T. Zhang

author

J. Zhou

author

Z. Ren

• Document Type: ARTICLE
• Publication order reference: Z. Yang, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China