Trials of stimulation extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28

• Authors: A. Demczuk , J. Kowalewska-Piontas , W. Bednarski
• Languages of publication: PL

Abstracts

EN

To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28 yeasts, we used: glycin, L-asparagine, L-leucine, dimethylformamide, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycin was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.

Keywords

EN

BETA-GALACTOSIDASE; KLUYVEROMYCES FRAGILIS; SECRETION; YEAST

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 1
• Pages: 253-259

Contributors

author

A. Demczuk

author

J. Kowalewska-Piontas

author

W. Bednarski

• Document Type: ARTICLE
• Publication order reference: A. Demczuk, Katedra Biotechnologii Zywnosci, Wydzial Nauki o Zywnosci, Uniwersytet Warminsko-Mazurski, ul. Heweliusza 1, 10-759 Olsztyn-Kortowo, Polamd