Trials of stimulation extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28
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To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28 yeasts, we used: glycin, L-asparagine, L-leucine, dimethylformamide, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycin was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.
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A. Demczuk, Katedra Biotechnologii Zywnosci, Wydzial Nauki o Zywnosci, Uniwersytet Warminsko-Mazurski, ul. Heweliusza 1, 10-759 Olsztyn-Kortowo, Polamd