Preparation of 13C- and 15N-labelled RNA for the structural atudy using multi-dimensional, heteronuclear magnetic resonance techniques
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The article briefly presents ways of RNA isolation from isotopically labelled bacteria and its subsequent degradation to 13C- and 15N-labelled nucleoside 5'-monophosphates then enzymatically converted to NTPs. 13C- and 15N-labelling of RNA is achieved by in vitro transcription with T7 RNA polymerase using isotopically labelled nucleoside 5'-triphosphates as a substrates and DNA template.
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