Preparation of 13C- and 15N-labelled RNA for the structural atudy using multi-dimensional, heteronuclear magnetic resonance techniques

• Authors: M. Olejniczak
• Languages of publication: PL

Abstracts

EN

The article briefly presents ways of RNA isolation from isotopically labelled bacteria and its subsequent degradation to 13C- and 15N-labelled nucleoside 5'-monophosphates then enzymatically converted to NTPs. 13C- and 15N-labelling of RNA is achieved by in vitro transcription with T7 RNA polymerase using isotopically labelled nucleoside 5'-triphosphates as a substrates and DNA template.

Keywords

EN

HETERONUCLEAR NMR   IN VITRO RNA SYNTHESIS   RNA STRUCTURE  

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1996
• Issue: 1
• Pages: 176-182
• Document Type: article
• Publication order reference: M.Olejniczak