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Journal
2009 | 1 | 86-97
Article title

Production of proteins in the cell-free expression systems

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Languages of publication
PL
Abstracts
EN
Efficient protein synthesis has become a critical issue in recent biotechnology and functional protemic studies. Traditional expression of protein performed in host cells such as Escherichia coli or Saccharomyces cerevisiae is generally lengthy and costly. Cell-free protein synthesis is an attractive alternative offering simplicity and fast rate of the reaction as well as the generation of functional proteins that are difficult to obtain using in vivo systems. Furthermore, the open nature of these systems makes it amenable to manipulation allowing the investigations into the mechanism of protein synthesis itself and into the inhibition of that process by interfering molecules such as antibiotics. Here we review all the main classes of cell-free protein expression system and we emphasize their potency and recent applications in biotechnology.
Journal
Year
Issue
1
Pages
86-97
Physical description
Contributors
author
author
author
author
References
Document Type
REVIEW
Publication order reference
Witold Szafarski, Katedra i Zaklad Histologii i Embriologii, Uniwersytet Medyczny im. K. Marcinkowskiego, ul. H. Swiecickiego 6, 60-781 Poznan, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-525d358f-0250-3c50-819c-2363da859bd0
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