Micropropagation of Salvia officinalis L. by shoot tips
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In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 ?mol/l IAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 ?mol/l was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was ? MS agar medium without growth regulators.
Publication order reference
I. Grzegorczyk, Zaklad Biologii i Botaniki Farmaceutycznej, Uniwersytet Medyczny, ul. Muszynskiego 1, 90-151 Lodz, Poland