PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2007 | 55 | 1-2 | 47-51
Article title

Effect of hibernation on sodium and chloride ion transport in isolated frog skin

Title variants
Languages of publication
EN
Abstracts
EN
The aim of the study was to evaluate the effect of hibernation on electrophysiological parameters of isolated frog skin under control incubation (Ringer solution) and after inhibition of Na+ and Cl- transepithelial transport by application of amiloride and bumetanide. The transepithelial electrical potential difference (PD in mV) was measured before and after mechanical stimulation of isolated frog skin. The tissues were mounted in a modified Ussing chamber. The results revealed a reduced PD of frog skin during hibernation. In February, as compared with November, PD of frog skin incubated in Ringer solution decreased by about 50%. Hibernation also affected hyperpolarization (dPD) of frog skin after mechanical stimulation. In November and December, dPD was about 50% and 30% lower, respectively, compared with the subsequent two months of the experiment. The incubation of frog skin with amiloride, a sodium ion channel blocker, resulted in reduced values of all measured electrophysiological parameters irrespective of the phase of hibernation. After application of chloride ion transport inhibitor (bumetanide), the PD in November and December decreased compared with the control incubation by about 80% and 75%, while in January and February by about 40% and 25%, respectively. In January and February dPD increased by four times and three times as compared with November and December. Hibernation reduces net ion flow in isolated frog skin. During the initial period of hibernation the sensitivity of the skin to mechanical stimulation also decreases. Towards the end of hibernation, on the other hand, excitation of mechanosensitive ion channels takes place.
Keywords
Publisher

Journal
Year
Volume
55
Issue
1-2
Pages
47-51
Physical description
Contributors
References
Document Type
ARTICLE
Publication order reference
Danuta Kosik-Bogacka, Chair and Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-50552212-82ef-3c78-9594-e8a77b9695ec
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.