Cytological investigations of the interspecific hybrids of Nicotiana tabacum L. ? N. glauca Grah.

• Authors: A. Trojak-Goluch , A. Berbec
• Languages of publication: EN

Abstracts

EN

Interspecific amphihaploid and amphidiploid hybrids between Nicotiana glauca Grah. (2n = 24) and N. tabacum L. (2n = 48) cultivars BY 103 and K 326 were analysed. F1 amphihaploids (2n = 36) were viable and completely self- and cross-sterile, and mostly univalents were present during meiosis (with pairing range from 0 to 5). In some meiocytes, meiotic irregularities were observed, such as sporadic chromatin bridges and formation of restitution nuclei. The resultant F1 hybrids were easily converted to amphidiploids (2n = 72) via colchicine treatment of seedlings. The number of univalents and the frequency of PMCs containing unpaired chromosomes indicated that amphidiploids N. tabacum cv. BY 103 or K 326 ? N. glauca represented quite a high pairing category. However, they were male sterile because pollen mother cells were arrested at the tetrad stage. The termination of development of PMCs, and consequently male sterility, are very rare in this kind of tobacco hybrids.

Keywords

EN

AMPHIDIPLOID; AMPHIHAPLOID; INTERSPECIFIC HYBRIDIZATION; MALE STERILITY; NICOTIANA GLAUCA; NICOTIANA TABACUM

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2003
• Volume: 44
• Issue: 1
• Pages: 45-54

Contributors

author

A. Trojak-Goluch

author

A. Berbec

• Document Type: ARTICLE
• Publication order reference: A. Trojak-Goluch, Institute of Soil Science and Plant Cultivation, Special Crops Department, ul. Czartoryskich 8, 24-100 Pu?awy, Poland