For the development of aseptic culture, Dendrobium kingianum Bidwill orchid pseudobulbs (2.0-3.0 cm long) with one or two terminal leaves were surface sterilized with 0.1% mercuric chloride solution and transferred to initial MS medium, supplemented with auxin IAA at 0.5 mg dm-3 and cytokinin BA at 1.0 mg dm-3. New shoots, which developed after 4-6 weeks of culture at the base of pseudobulbs or at their upper nodes, were transferred to basal MS medium suplemented with an auxin (IAA or NAA) at 0.5 and 1.0 mg dm-3 and cytokinin Kinetin or 6-benzylaminopurine (BA) at 0.5, 1.0 and 2.0 mg dm-3 in different combinations. The responses of orchid cultures varied according to concentrations and type of growth regulators. From tested growth regulators, BA in concentration 2.0 mg dm-3 in combination with 0.5 mg dm-3 IAA was the most effective for shoot induction. At 0.5 mg dm3 both IAA and NAA strongly stimulated shoots multiplication in comparison to 1.0 mg dm-3. NAA-BA combinations positively influenced the shoot length. For roots induction, the NAA-Kinetin combinations were better than the remaining ones. Kinetin stimulated the increase of the root length.