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The unique thermotolerance of hyperthermophiles, suitable as valuable sources of thermostable enzymes, is a result of various minor changes which led to the restriction of protein flexibility and modifications of nucleic acids and membrane lipids. Furthermore, all hyperthermophiles produce a number of heat shock proteins and thermoprotectants. Thermal resistance of these microorganisms is also enhanced by rapid resynthesis of thermolabile compounds and by elimination of such intermediates from cell metabolism.