Production of secondary metabolites in shoots of Salvia nemorosa L. cultured in vitro
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The shoots of Salvia nemorosa were proliferated in vitro from shoot tips of 4-week-old seedlings on Murashige and Skoog (MS) agar (0.7%) medium containing indolilo-3-acetic acid (IAA) 0.5 mg/l and benzylaminopurine (BAP) 1 mg/l. Four sterols (campesterol, stigmasterol, beta-sitosterol, stigmastanol) and two triterpenes (ursolic acid, oleanolic acid) were detected by GC-MS method in the chloroform extract obtained from the dry in vitro cultured shoots of S. nemorosa. From the methanolic extract, rosmarinic acid both as isomer trans and cis was isolated and identified on the basis of 1H-NMR and HH-COSY spectral data. It is the first report on the detection of cis-rosmarinic acid in plants.
Publication order reference
L. Kuzma, Zaklad Biologii i Botaniki Farmaceutycznej, Katedra Biologii i Biotechnologii, Uniwersytet Medyczny, ul. Muszynskiego 1, 90-151 Lodz, Poland