An attempt at the transformation of embryogenic genotypes of alfalfa using gene pPR97/GUS/Intron-LLpr10.1a promoter
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The transformation was led by pPR97/GUS/Intron LLpr10.1a promoter gene, which codes the acid protein. Two different media were used in the experiment. On Blayds medium, the highest efficiency of soamatic embryogenesis (25%) was observed in the Kometa variety. The culture media developed by Guelph University appeared to be the best for the Kama variety (18%).
Publication order reference
Z. Broda, Katedra Genetyki i Hodowli Roslin, Akademia Rolnicza, ul. Wojska Polskiego 71c, 61-625 Poznan, Poland