An attempt at the transformation of embryogenic genotypes of alfalfa using gene pPR97/GUS/Intron-LLpr10.1a promoter

• Authors: Z. Broda , J. Morawska
• Languages of publication: PL

Abstracts

EN

The transformation was led by pPR97/GUS/Intron LLpr10.1a promoter gene, which codes the acid protein. Two different media were used in the experiment. On Blayds medium, the highest efficiency of soamatic embryogenesis (25%) was observed in the Kometa variety. The culture media developed by Guelph University appeared to be the best for the Kama variety (18%).

Keywords

EN

IN VITRO CULTURE; SOMATIC EMBRYOGENESIS; TRANSFORMATION

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2000
• Issue: 4
• Pages: 106-111

Contributors

author

Z. Broda

author

J. Morawska

• Document Type: ARTICLE
• Publication order reference: Z. Broda, Katedra Genetyki i Hodowli Roslin, Akademia Rolnicza, ul. Wojska Polskiego 71c, 61-625 Poznan, Poland