Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl

PL EN


Preferences help
enabled [disable] Abstract
Number of results
2010 | 51 | 1 | 1-8

Article title

Possible ancient origin of heterochromatic JNK sequences in chromosomes 2R of Secale vavilovii Grossh

Title variants

Languages of publication

EN

Abstracts

EN
Employing FISH analysis as well as BLAST and CUSTAL W (1.82) programs, we investigated types of DNA nucleotide sequences building an additional heterochromatic band in 2R chromosomes of 3 lines of Secale vavilovii Grossh. The probes used in FISH analysis were designed based on the reverse transcriptase sequence of Ty1-copia and Ty3-gypsy retrotransposons and the 5S rRNA gene sequence. No hybridization signals from the reverse transcriptase probes were observed in the chromosome region where the additional band occurs. On the other hand, signals were observed after hybridization with the 5S rDNA probe, clearly suggesting the presence of that type of sequences in the analyzed heterochromatin band. Using BLAST and CUSTAL W programs, we revealed high similarity of the JNK1 sequence to the 5S rRNA gene from Hordeum chilense (HCH1016, HCH1018, 88%) and to a fragment of the 5S rRNA sequence of H. marinum (HMAR003, 97%). In addition, the same fragment of JNK1 was shown to be very similar to the part of the Angela retrotransposon (92%) as well as to the SNAC 426K20-1 transposon (89%) belonging to CACTA family, both from Triticum monococcum, and to Zingeria biebersteiniana pericentromeric sequences (78%). The similarity of JNK1 to those sequences may be accidental or the JNK1 may represent an ancient mobile genetic element that caught the 5S rRNA sequence. During the evolution those sequences might have been accumulated in the particular region on the 2R chromosome. Our results suggest that the additional heterochromatin band in chromosomes 2R of S. vavilovii is a collection of defective genes and/or mobile genetic elements.

Discipline

Year

Volume

51

Issue

1

Pages

1-8

Physical description

Contributors

author
author
author

References

Document Type

ARTICLE

Publication order reference

M. Achrem, University of Szczecin, Faculty of Natural Sciences, Chair of Cell Biology, Waska 13, 71? 415 Szczecin, Poland

Identifiers

YADDA identifier

bwmeta1.element.element-from-psjc-4c08fd3c-2d16-3b99-8d12-2d3fafc26311
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.