Mechanism of carnitine transport catalyzed by carnitine carrier from rat brain mitochondria
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The tramnsport mechanism of reconstituted carnitine carrier purified from rat brain mitochondria was studied kinectically.Short and medium chain acyl carnitine derivatives had much higher affinity to the carnitine carrier in comparison with long chain acyl carnitine derivatives, therefore both homologous (carnitine/carnitine) and heterologous (carnitine/acetylcarnitine) antiports were analysed. A complete set of half-saturation constans was established for various subsrtrate concentrations on both the external and internal side of the membrane.Bisubstrate initial velocity analyses of the exchange reaction in a kinetic pattern which is consistent with a sequential antiport mechanism.This type of mechanism implies formation of a ternary complex of the carrier with one internal and one external substrate molecule before the transport reaction occurs.
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