The subject of the study was fertile diploid hybrids of rye, cv. Amilo (2n = RR = 14) with Dasypyrum villosum (Crimea, Ukraine) (2n = VV = 14) ? wild species belonging to Triticeae. translocation. Plants which morphologicaly resembled rye and which showed the highest The analysed strains were obtained using in vitro cultures with subsequent backcrossing of hybrid plants with pollen of ?Amilo? or D. villosum in the case of heterozygotic fertility and total protein content in kernels were selected during several generations. The small differences in the occurrence of heterochromatine bands in rye chromosomes were revealed by the differential staining technique at the stage of mitosis, between hybrid plants and ?Amilo? cv. Cells with mixoploid chromosome number 2n = 13, 2n = 14 and 2n = 15 were present in some plants. Neither substitution nor evident chromosome translocation were found in generations B3/F3 and B4/F2 under investigation. Therefore, the technique of nucleic acids hybridization in situ will be applied to detect fragments of D. villosum genotype introgressed into rye.