From the economical point of view alfalfa is a very valuable plant. It has very high yield potential, it is rich in protein, vitamins and minerals, so it is prized as very important feed for horses, beef cattle, sheep, goats and other domestic animals. That is why the improving of alfalfa quality by the methods of molecular biotechnology is a very interesting issue. In this review information about the crucial elements of alfalfa transformation and regeneration are gathered together. Among other following elements, genetic background of plant, A. tumefaciens strains, explant types, in vitro culture medias, plant development stages are discussed. It seems that optimalisation of the transformation and regeneration procedure are individual for every plant genotype and A. tumefaciens strain.