Effects of phenylhydrazine or recombinant human erythropoietin on deformability and activity of dehydrogenase glucose-6-phosphate and acetylcholinesterase in Wistar rats blood enriched in reticulocytes

• Authors: E. Nowak , G. Wyrwicz , Z. Dabrowski , O. Smolenski , K. Spodaryk
• Languages of publication: EN

Abstracts

EN

Deformability and activity of the enzymes: acetylcholinesterase (AChE) and dehydrogenase glucose-6-phosphate (G-6-PD), were assayed for RBC enriched in immature reticulocytes. Reticulocytosis was evoked by administration of two different drugs: recombinant human erythropoietin (rHuEPO) and phenylhydrazine (PHZ) to two groups of Wistar rats. After treatment with the former compound, a group of animals exhibited 17,33 % reticulocytes in blood whereas a group of rats treated with the latter drug reached 57,66 % of these cells in blood. A marked decrease in RBC deformability was found in both groups of animals. AChE did not significantly change activity neither in PHZ-treated nor in rHuEPO-treated rats, whereas G-6-PD activity was significantly decreased in the PHZ-treated group.

Keywords

EN

ACETYLCHOLINESTERASE; DEFORMABILITY; DEHYDROGENASE GLUCOSE-6-PHOSPHATE; ERYTHROPOIETIN; PHENYLHYDRAZINE; RETICULOCYTOSIS

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2003
• Volume: 51
• Issue: 3-4
• Pages: 195-199

Contributors

author

E. Nowak

author

G. Wyrwicz

author

Z. Dabrowski

author

O. Smolenski

author

K. Spodaryk

• Document Type: ARTICLE
• Publication order reference: Z. Dabrowski, Department of Hematology and Toxicology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Krakow, Poland