RAPD (Randomly Amplified Polymorphic DNA) and glutenin SDS-PAGE analyses were performed on wheat hybrid strains derived from multiple crosses of hybrids between Ae. ventricosa and T. durum with hexaploid wheat. We found Aegilops specific bands (G03580 and T02990) in two hybrid strains (VGPB and VGPAA) using RAPD method. The presence of Ae. ventricosa specific fraction of glutenin was shown in one wheat hybrid strain (VGPP).