Karyotypic details were studied in males of six beetle species from three families, viz. Tenebrionidae: Dailognatha pumila Bdy. (2n=20, n male= 9+Xyp), Pachyscelis musiva Menetr. (2n=18, n male= 8+Xyp), Pimelia capito Kryn. (2n = 18, n male= 8+Xyp); Cerambycidae: Agapanthia walteri Reitt. (2n=20, n male = 9+Xyp), Agapanthia korostelevi Danilevsky (2n=20, n male = 9 + Xyp); Curculionidae: Phyllobius caucasicus Stierl. (2n = 22, n male=10+Xyp). The chromosome number and sex determining system of all beetle species are described for the first time. Evolutionary trends in karyotypes of the studied beetle groups are briefly discussed.