The aim of the present research was the assessment of efficiency of micropropagation system for selected species that belong to Asteraceae family, as well as analysis of morphological traits and plantlets ability to flower. The experimental material were shoot tips isolated from 7-day-old seedlings of Leontopodium alpinum, Senecio macrophyllus, Carlina acaulis, and Cirsium pannonicum. The shoot tips were transferred on the medium supplemented with BA (1 mg.dm-3), and NAA (0,1 mg.dm-3). The obtained shoots were transferred onto fresh medium with the same combination of growth regulator (3 subcultures). The shoots with 4 or more leaves were rooted on the half-strength MS medium without growth regulators for 4 weeks. The plantlets were acclimatized to ex vitro conditions and planted to the field. The analysis of flowering ability, leaves and flower morphology, and survival level were the objectives of the study. The plantlets were acclimatized in a greenhouse and planted to the field condition. From 10 seeds of initial material one can obtain 24 278 plants of Leontopodium alpinum, 1507 of Carlina acaulis, 1261 of Senecio macrophyllus, and 37 of Cirsium pannonicum taking into consideration the percentage of seed germination, proliferation rate in three subcultures, frequency of microshoots rooting and survival rate during acclimatization. The regenerated plants demonstrated traits of donor plants and were able to flower and produce viable seeds.