Anthocyanin accumulating cell lines were established from storage root, leaf and stem explants of sweetpotato (Ipomoea batatas (L.) Lam.), cv. Ayamurasaki. The calli developed on MS basal medium enriched with 2,4-D at 27?C accumulated pigments in the dark. The storage root originated suspension culture generated the highest amount of total anthocyanins expressed as colour value (5.9) followed by the cultures originating from leaf (4.3) and stem (1.7). The cultures displayed similar anthocyanin profile regardless of source of explants. The major pigments derived from suspension cultures appeared with earlier retention time on ODS-column HPLC than the YGM pigments accumulated in vivo, which suggests that they are highly hydrophilic and have simpler chemical structure.