Micropropagation of Leucojum aestivum L. in bioraector RITA?
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Embryogenic callus of Leucojum aestivum was transferred to solid and liquid media in bioreactor RITA? vessels. Callus growth on the liquid medium enriched with 2 and 5 M Picloram and 0.5 M BA was characterized by a higher multiplication index. The greatest number of somatic embryos was observed on the callus multiplied in bioreactor using 5-minute flushing with the medium. Globular embryos developed into torpedo-stage embryos and plantlets under the influence of NAA (0.5 M) and zeatin (5 M). Liquid medium promoted the development of L. aestivum somatic embryos and plantlets.
Publication order reference
Agata Ptak, Katedra Hodowli Roslin i Nasiennictwa, Uniwersytet Rolniczy, ul. Lobzowska 24, 31-140 Krakow, Poland