Embryo development after interspecific hybridization of Lupinus albus L., L. mutabilis Sweet. and L. angustifolius L.
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Embryo development of Lupinus albus L. and L. mutabilis Sweet. after pollination with L. angustifolius L. was studied. Observations of ovules revealed non typical embryo development. From the 5th day after pollination, high number of degenerated ovules was found; the highest number was observed on 15 and 20 days after pollination. The period between the 10th and 15th day after pollination was the most suitable for isolation of embryos in all the examined combinations, with exception L. albus x L. angustifolius where optimal isolation time was between 15th and 20 th day after pollination. Embryos were obtained in each cross pollination. Some of them developed in vitro into plantlets and grew in an artificial medium for some time, but most of them died due to non typical development of the root system, stem, or both. The hybrid character of the survived plants requires confirmation.
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J.A.Przyborowski, Department of Plant Breeding and Seed Production, University of Agriculture and Technology, Plac Lodzki 3, 10 724 Olsztyn, Poland