Sperm cells as DNA vectors for production of transgenic animals
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The transgenic animals are obtained mainly by microinjection of exogenous DNA into male pronuclei of the zygote. The efficiency of this method is not satisfactory, especially in domestic animals. Using the sperm cells as vectors for transfering exogenous DNA sequences into the oocyte during the process of fertilization seems to be an easy and simple method for production of transgenic animals. It was discovered that sperm cells of all species show the spontaneous ability to take up exogenous DNA. Exogenous DNA binding is not accidental, it always occours in the postacrosomal segment of the sperm head. The interaction is ionic, reversible, sequence ? independent, and not restricted to DNA, but can be reproduced using other negetively charged macromolecules. The introduction of DNA into sperm cells is possible using different methods, however, any of these methods could be considered as a routine one. The mechanisms of taking up and associationig DNA by sperm are being constantly revealed and better understood.
Publication order reference
Joanna Jurkiewicz, Dzial Biotechnologii Rozrodu Zwierzat, Instytut Zootechniki, 32-083 Balice k. Krakowa, Poland