The influence of reaction conditions on synthesis of esters by Mucor javanicus T45 lipase
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The effect of reaction milieu on synthesis of esters of higher fatty acids by Mucor javanicus T45 lipase was investigated. The folloving factors were studied: a temperature, an organic solvent, concentrations of substrates and their molar ratios, a solvent concentration, pH of water present in the lipase preparation and a concentration of diethanoloamine (DEtA) applied to modifity the essential water layer of the enzyme. It was revelated that M. javanicus lipase, immobilized in situ in its mycelium, preserves from 48 to 92 percent of the initial activity after heating for 1 houer at 100oC in nonpolar solvents. The modification of the enzyme essential water layer with DEtA increases the synthetic activity of the enzyme by about 70 percent.
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