Detection of bovine leukocyte adhesion deficiency (BLAD) carriers using a new PCR test

• Authors: S. Kaminski , U. Czarnik
• Languages of publication: EN

Abstracts

EN

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

Keywords

EN

BLAD   CATTLE   PCR  

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 1
• Pages: 51-55
• Document Type: article
• Publication order reference: S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, M. Oczapowskiego 5/030, 10-718 Olsztyn, Poland.