Detection of bovine leukocyte adhesion deficiency (BLAD) carriers using a new PCR test

• Authors: S. Kaminski , U. Czarnik
• Languages of publication: EN

Abstracts

EN

In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.

Keywords

EN

BLAD; CATTLE; PCR

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 1
• Pages: 51-55

Contributors

author

S. Kaminski

author

U. Czarnik

• Document Type: article
• Publication order reference: S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, M. Oczapowskiego 5/030, 10-718 Olsztyn, Poland.