Detection of bovine leukocyte adhesion deficiency (BLAD) carriers using a new PCR test
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In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.
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S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, M. Oczapowskiego 5/030, 10-718 Olsztyn, Poland.