Polymorphism of nine canine-derived microsatellites (CPH1, CPH3, CPH6, CPH11, 2004, 2010, 2140,2168 and 2319) was studied in a group of 91 unrelated silver foxes kept on a commercial farm. Among the studied microsatellites two appeared to be dimorphic (CPH1 and 2140) and another two (2010 and 2319) were highly polymorphic, with PIC (Polymorphic Information Content) values of 0.775 and 0.692, respectively. Other five microsatellites demonstrated medium polymorphism and the PIC values ranged from 0.548 to 0.616. It was calculated that if all the studied markers were applied for paternity testing, then combined exclusion probability would be 0.989. Microsatellite polymorphisms in the silver fox, blue fox and dog were compared and tendency toward longer alleles in the dog was revealed. It was confirmed that canine-derived microsatellites can be successfully applied for parentage control and genome mapping in silver foxes.