Embryonic birds? cell manipulation
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Because of easy access to the developing embryo in the laid eggs, chicken embryos have been used as a favorite model in embryology and developmental biology. Several experiments have demonstrated that pluripotent blastodermal cells (BC) from stage X chicken embryo can be removed, dispersed, and injected into the subgerminal cavity of recipient embryos at the same stage of development. These blastodermal cells are able to colonize somatic tissues of treated embryos and can contribute to the germline, because stage X blastodermal cells obtained from freshly laid eggs contain primordial germ cells (PGC) or their precursor cells. In this overview the methods and two principal applications of chicken embryo cells manipulation are: 1) production of transgenic birds, and 2) preserving and reconstituting poultry foundation stocks were presented.
Publication order reference
M. Bednarczyk, Oddzial Badawczy Drobiarstwa, Instytut Zootechniki, Zakrzewo k. Poznania, 62-069 Paledzie, Poland