Purification and properties of chitin deacetylase from Absidia orchidis

• Authors: K.W. Szewczyk , M. Orzeszak
• Languages of publication: PL

Abstracts

EN

Methods of purification of chitin deacetylase are discussed. A two step method of purification of chitin deacetylase from mycelial extracts of the fungus Absidia orchidis by chromatography is presented. The crude enzyme extract was purified by a gel chromatography and then by ion exchange chromatography. Specific activity of purified enzyme was 12.3 U/mg and final purification degree was 147. The apparent molecular mass of the enzyme was 75 kDa. When O ? hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pH for enzyme activity was 5,5 and the optimum temperature was 50?C.

Keywords

EN

CHITIN DEACETYLASE; PURIFICATION

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2002
• Issue: 2
• Pages: 48-59

Contributors

author

K.W. Szewczyk

author

M. Orzeszak

• Document Type: REVIEW
• Publication order reference: K. W. Szewczyk, Wydzial Inzynierii Chemicznej i Procesowej, Politechnika Warszawska, ul. Warynskiego 1, 00-645 Warszawa, Poland