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Journal
2002 | 2 | 48-59
Article title

Purification and properties of chitin deacetylase from Absidia orchidis

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PL
Abstracts
EN
Methods of purification of chitin deacetylase are discussed. A two step method of purification of chitin deacetylase from mycelial extracts of the fungus Absidia orchidis by chromatography is presented. The crude enzyme extract was purified by a gel chromatography and then by ion exchange chromatography. Specific activity of purified enzyme was 12.3 U/mg and final purification degree was 147. The apparent molecular mass of the enzyme was 75 kDa. When O ? hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pH for enzyme activity was 5,5 and the optimum temperature was 50?C.
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Journal
Year
Issue
2
Pages
48-59
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author
References
Document Type
REVIEW
Publication order reference
K. W. Szewczyk, Wydzial Inzynierii Chemicznej i Procesowej, Politechnika Warszawska, ul. Warynskiego 1, 00-645 Warszawa, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-2a47a62f-0969-31ec-8e2e-f34d193f15d9
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