Resistance to antibiotics of the selected species of Agrobacterium rhizogenes
Languages of publication
Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.
Publication order reference
E. Lojkowska, Mi?dzyuczelniany Wydzial Biotechnologii, Uniwersytet GdaNski i Akademia Medyczna, ul. Kladki 24, 80-822 Gdansk, Poland e-mail: firstname.lastname@example.org