Lack of relationship between serum content of MBL, sCD14, anti-PPD and anti-Hsp65 IgG, and ingestion of Mycobacterium bovis BCG bacilli by phagocytes

• Authors: B. Paziak-Domanska , A. Bonar , M. Kowalewicz-Kulbat , M. Klink , M. Kowalski , J. Karhukorpi , R. Karttunen , M. Jurkiewicz , B. Rozalska , W. Rudnicka
• Languages of publication: EN

Abstracts

EN

Prophylactic vaccination against tuberculosis (TB) with a live attenuated strain of Mycobacterium bovis Bacille Calmett?e-Gerin (BCG) has been used worldwide. However, TB remains one of the most significant diseases of humans and animals. Better understanding of the mechanisms of human immunity to mycobacteria is essential for development of new vaccines and estimation of their efficacy. In this study we determined the levels of known humoral mediators of mycobacterial phagocytosis - mannose binding lectin (MBL), soluble CD14 (sCD14), antibodies of IgG class against mycobacterial purified protein derivative (PPD) and mycobacterial Hsp65 antigen, in the sera from healthy young volunteers vaccinated with BCG and presenting positive and negative Mantoux responses to PPD. Than we asked a question as to whether macrophages and polymorphonuclear leukocytes (PMNs) from the individuals with positive (TT(+)) and negative (TT(-)) tuberculin tests differ by the ability to ingest mycobacteria. Also we were looking for a relation between the intensity of mycobacterial ingestion by phagocytes in the medium with autologous sera containing different concentration of MBL, sCD14 andf anti-mycobacterial IgG. We found no significant differences between the investigated parameters for TT(+) and TT(-) volunteers. Our result suggest that ability of macrophages and PMNs to ingest mycobacteria depends on an individual intrinsic capacity of phagocytes.

Keywords

EN

MACROPHAGE; POLYMORPHONUCLEAR LEUKOCYTE; TUBERCULIN TEST

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2002
• Volume: 50
• Issue: 5
• Pages: 337-344

Contributors

author

B. Paziak-Domanska

author

A. Bonar

author

M. Kowalewicz-Kulbat

author

M. Klink

author

M. Kowalski

author

J. Karhukorpi

author

R. Karttunen

author

M. Jurkiewicz

author

B. Rozalska

author

W. Rudnicka

• Document Type: ARTICLE
• Publication order reference: B. Paziak-Domanska, Department of Immunology and Infectious Biology, University of Lodz, Banacha 12, 90-237 Lodz, Poland