Regeneration of summer snowflake (Leucojum aestivum L.) in in vitro cultures
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Slices of scales and leaves isolated from Leucojum aestivum L. bulbs were cultured in the presence of auxins (NAA, 2,4-D, Picloram) and cytokinin, BA at different proportions and concentrations. The medium free of growth regulators was also used. Regeneration occurred as a result of somatic embryogenesis and organogenesis. Explants cultivated on the media containing 2,4-D and Picloram showed a capacity of somatic embryogenesis, viz. formation of embryogenic callus and somatic embryos. On the other hand, direct organogenesis (induction of bulblets and roots) occurred in explants grown on the medium devoid of growth regulators and enriched in NAA.
Publication order reference
A. Ptak, Chair and Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Krakow, Poland