The effect of transformation with iaglu gene on growth and development of trangenic strawberry in in vitro cultures

• Authors: D. Wawrzynczak , I. Sowik , L. Michalczuk
• Languages of publication: PL

Abstracts

EN

The aim of the presented work was to study the effects of changes of endogenous indole-3-acetic acid (IAA) metabolizm on in vitro shoot proliferation and rhizogenesis of transgenic strawberry shoots carrying maize IAA-glucose synthase gene (iaglu). Four iaglu-transformed strawberry clones and nontransformed 'Kaster' shoots served as a plant material for the study. The analysis of free and conjugated IAA level in leaves of transgenic and control strawberry plants showed that iaglu-containing strawberry clones had significantly higher level of ester conjugated IAA, but the level of free hormone was only slightly decreased or comparable to the control plants. iaglu-transformed clones had significantly higher proliferation rate and formed more roots than the control shoots. One of the iaglu-transformed clones had significantly shorter and other two ? longer roots than the control plantlets.

Keywords

EN

AUXIN; GENETIC TRANSFORMATION; HOMEOSTASIS; PROLIFERATION; ROOTING; STRAWBERRY

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 46-53

Contributors

author

D. Wawrzynczak

author

I. Sowik

author

L. Michalczuk

• Document Type: ARTICLE
• Publication order reference: D. Wawrzynczak, Zaklad Fizjologii i Biochemii Roslin, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland