Enhanced responsiveness of rat cardiac myocytes to muscarinic cholinergic stimulation during chemically-induced hypoxia

• Authors: Michal Majewski , Hanna Laskowska-Bozek , Joseph A. Moutris , Slawomir Maslinski , Jan Ryzewski
• Languages of publication: EN

Abstracts

EN

In contrast to adrenaline, exogenously administrated cholinergic agonist, carbachol have very little effect on the contractility of rat cardiac myocytes, unless its contracitle has been increased by adrenergic agonist. This interaction between the muscarinic and adrenergic pathways has been suggested to be the major means by which muscarinic agonist alters adrenergic function. When the cardiac myocytes were incubated in the medium contained the mitochondrial respiratory inhibitor potassium cyanide (chemically-induced hypoxia)the spontaneous contractility was ceased. The contractility partly recovered that during chemical hypoxia, in which cellular ATP is decreased (37% of control), the respponsiveness of myocytes to muscarinic cholinergic stimulation significantly increase. Contraction of myocytes, stimulated by adrenaline was inhibited by carbachol in control cells as well as in cells with chemically-induced hypoxia. This increase in physiological response to muscarinic stimulation was associated with an increase of muscarinic receptors (630%). The results support the hypothesis that in ischaemic/hypoxic myocardium the role of cholinergic system may be more important than previously assumed.

Keywords

EN

ATP; HYPOXIA; MUSCARINIC CHOLINERGIC RECEPTOR; MYOCYTE

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 1993
• Volume: 53
• Issue: 1
• Pages: 79-91

Contributors

author

Michal Majewski

author

Hanna Laskowska-Bozek

author

Joseph A. Moutris

author

Slawomir Maslinski

author

Jan Ryzewski

• Document Type: paper