PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2006 | 1 | 68-81
Article title

The use of new strategies in the cloning studies on somatic cell cloning in pigs

Title variants
Languages of publication
PL
Abstracts
EN
Somatic cell nuclear transfer (SCNT) technique in pigs remains relatively low (2% to 5% of produced piglets), that is why further efforts have to be made to optimize both a multi-step cloning procedure and to improve a structuro-functional quality of recipient oocytes and nuclear donor cells. Pre- and postimplantation developmental potential of porcine SCNT-derived embryos depends to a high degree on not only coordination of mitotic cycle stage with phenotype of nuclear donor cell, but also proper combination of the methods of maternal chromosome elimination (enucleation), oocyte reconstruction techniques, the systems of artificial activation of generated nuclear-cytoplasmic hybrids (clonal cybrids) and in vitro culture of reconstructed embryos. Generally, it can result in increasing the competences of both somatic nuclear and mitochondrial genome for epigenetic remodeling/reprogramming in developing cloned embryos.
Publisher

Journal
Year
Issue
1
Pages
68-81
Physical description
Contributors
author
References
Document Type
REVIEW
Publication order reference
Maria Skrzyszowska, Dzial Biotechnologii Rozrodu Zwierzat, Instytut Zootechniki, 32-083 Balice k. Krakowa, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-25e2e5b3-aef4-37d5-b6ee-6afe321b91ad
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.