The effect of cytokinins on in vitro morphogenesis of sugar beet (Beta vulgaris L.)
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The aim of the studies was to estimate the effect of cytokinins on the production of callus and shoots from different seedling explants of sugar beet. Two diploid multigerm genotypes were used in the experiment. The seedling explants (hypocotyls, cotyledons and leaves) were cultured on MS medium supplemented with cytokinins: BAP, 2iP, TDZ in concentrations 0,2; 1,0 and 2,0 mg/l. Hypocotyls, cotyledons and leaves showed different abilites to form callus and shoots. After two weeks of culture, the induction of callus was mainly observed on the explants of hypocotyls and cotyledons, whereas adventitious shoots formed on leaf explants. Frequency of callus and shoots induction was dependent on the origin of the explants, concentration and type of the cytokinins. The highest percentage of regeneration was obtained on the explants of young leaves, on medium containing TDZ (51,5%). The optimal concentration of cytokinins was 1 mg/l. Plant regeneration on seedling explants came only under direct organogenesis.
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M. Goska, Zaklad Genetyki i Hodowli Roslin, Instytut Hodowli i Aklimatyzacji Roslin, Powstancow Wielkopolskich 10, 85-090 Bydgoszcz, Poland