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Journal
2011 | 2 | 209-213
Article title

Embryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings

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Languages of publication
EN
Abstracts
EN
The study investigates the regeneration capacity of Crambe abyssinica via the callus phase. The conducted experiments provide an overview of various conditions appropriate for callus development and its subsequent differentiation. Explants from cotyledons and leaves of sterile 14-day-old seedlings of C. abyssinica cv. Mayer were used. The primary callus so obtained was subsequently utilised for the observation of embryogenesis. The best callus formation on the explants was achieved on the agar-solidified MS medium with 0.5 mg . dm-3 NAA and 0.5 mg . dm-3 BAP or TDZ. The formation of secondary embryogenic callus was most efficient on MS medium supplemented with 0.4 mg . dm-3 NAA and 0.3 mg . dm-3 of BAP.
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Journal
Year
Issue
2
Pages
209-213
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Contributors
author
author
References
Document Type
ARTICLE
Publication order reference
Tomasz Furmanek, Institute of Experimental Biology and Environmental Protection, Pomeranian University in S?upsk, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-242b30f6-ec5a-3922-a6bf-b141b407e8a2
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