Embryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings
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The study investigates the regeneration capacity of Crambe abyssinica via the callus phase. The conducted experiments provide an overview of various conditions appropriate for callus development and its subsequent differentiation. Explants from cotyledons and leaves of sterile 14-day-old seedlings of C. abyssinica cv. Mayer were used. The primary callus so obtained was subsequently utilised for the observation of embryogenesis. The best callus formation on the explants was achieved on the agar-solidified MS medium with 0.5 mg . dm-3 NAA and 0.5 mg . dm-3 BAP or TDZ. The formation of secondary embryogenic callus was most efficient on MS medium supplemented with 0.4 mg . dm-3 NAA and 0.3 mg . dm-3 of BAP.
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Tomasz Furmanek, Institute of Experimental Biology and Environmental Protection, Pomeranian University in S?upsk, Poland