Cellular aspects of regeneration in protoplast culture of yellow lupin (Lupinus luteus L.)
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Yellow lupin (Lupinus luteus), like many other Fabaceae species, exhibits strong recalcitrance to in vitro conditions of protoplast culture, where regeneration capacity is extremely limited, and only incipient mitoses are observed. In case of the restricted morphogenetic potential of yellow lupin protoplasts, special attention should be paid to the cellular mechanisms that control gaining of totipotency in culture. These are especially: the structure and functioning of new cell wall, elements of cytoskeleton, as well as some cell components of signaling properties. Detailed investigation on these structures and their behaviour in culture conditions may contribute to the understanding and bypassing of the recalcitrance problem in yellow lupin.
Publication order reference
Alina Wiszniewska, Katedra Botaniki i Fizjologii Roslin, Wydzial Ogrodniczy, Uniwersytet Rolniczy, Al. 29 Listopada 54, Krakow, Poland