Effect of IFN-on expression of HLA in bare-lymphocyte syndrome-like cell line HAJ

• Authors: I. Nowak , B. Pochron , A. Kozlowska , J. Dubis , P. Kusnierczyk
• Languages of publication: EN

Abstracts

EN

We compared HLA antigen expression on new B-lymphoblastoid cell line (B-LCL) HAJ with that on B-LCLs expressing normal HLA levels as well as on B-LCLs derived from bare lymphocyte syndrome (BLS) patients and in vitro mutated B-LCLs of BLS-like phenotype. HAJ cells had no expression of HLA class II and low expression of class I antigens similarly to some of BLS B-LCLs, although HAJ cell line was derived from lymphocytes of HLA class I- and class II-normally expressing donor. HAJ cells displayed B lymphocyte markers, surface immunoglobulin and CD19. Culture of HAJ cells in the presence of interferon gamma resulted in HLA class I antigen upregulation, but did not restore class II expression. The cell line HAJ may prove useful for studies on factors influencing HLA class I cell surface expression.

Keywords

EN

BARE-LYMPHOCYTE; EXPRESSION; HLA ANTIGEN

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2001
• Volume: 49
• Issue: 6
• Pages: 453-

Contributors

author

I. Nowak

author

B. Pochron

author

A. Kozlowska

author

J. Dubis

author

P. Kusnierczyk

• Document Type: ARTICLE
• Publication order reference: I. Nowak, Laboratory of Immunogenetics, Department of Clinical Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroc?aw, Poland