EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

• Authors: H.-Y. Wang , Y.-M. Wei , Z.-H. Yan , Y.-L Zheng
• Languages of publication: EN

Abstracts

EN

SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.

Keywords

EN

GENETIC DIVERSITY; TRITICUM DURUM

Discipline

• GENETICS: GENETICS

• Journal: Journal of Applied Genetics
• Year: 2007
• Volume: 48
• Issue: 1
• Pages: 35-42

Contributors

author

H.-Y. Wang

author

Y.-M. Wei

author

Z.-H. Yan

author

Y.-L Zheng

• Document Type: ARTICLE
• Publication order reference: Y.L. Zheng, Triticeae Research Institute, Sichuan Agricultural University, Ya'an, Sichuan 625014, China