Preparation of pBIN19-PGIP-based genetic construct for modification of strawberry genome to obtain genotypes resistant to grey mould
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Polygalacturonase inhibiting protein (PGIP) cDNA was isolated using RT-PCR from fruit of apple 'Golden Delicious' which was previously wounded and inoculated with Botrytis cinerea. The sequence of the cDNA showed high level of homology with the published sequence of PGIP-cDNA from apple and pear (99,7 and 97,3%) and low similarity with the sequence of cDNA from tomato (51,5%). The obtained cDNA was cloned and used for the binary construct creation. The introduction of the pBIN19-PGIP construct into strawberry plant genome seems to be a promising strategy to obtain a genotype resistant to B. cinerea, causal agent of grey mould ? the most economically important fungal disease of this species.
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M. Korbin, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland