The aim of research works carried out at Textile Research Institute in Lodz and the Institute of Technical Biochemistry of the Technical University of Lodz was testing the possibility of applying pectinolytic and cellulolytic enzymes complex (from Aspergillus niger IBT-90) in the pretreatment process of cotton woven fabrics. Optimal conditions of pectinolytic enzymes? biosynthesis and accompanying cellulolytic enzymes and hemicellulases were developed by suitable choice of cultivation culture composition. Cotton fabric treatment was done on laboratory scale using different amounts of pectinolytic enzymes. The activity of enzymes in the pretreatment of cotton fabrics was evaluated on the basis of changes of woven fabrics mass and water sorption capabilities after biotreatment. The influence of applied pectinolytic enzymes, enriched by cellulases, on the change of chemical composition and strength parameters of woven fabrics after biotreatment was also discussed. The evaluation of liquid sorption by cotton woven fabrics was presented, taking into account sorption coefficients.