Application of real-time PCR technique for validation of microarray experiment
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Microarray technology has recently reached the mainstream. It is widely applied in many biological fields of studies. Despite of its uncontested advantages microarray results have to be validated using independent methodology. For this purpose real-time PCR platform has become the most popular method of choice. The ability of PCR product measurement after each cycle is connected here with wide dynamic range of detection. This review is a summary of our knowledge on the technical issue of quantitative PCR utilization in microarray results confirmation.
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Agnieszka Ciesielska, Instytut Chemii Bioorganicznej, Polska Akademia Nauk, ul. Noskowskiego 12/14, 61-704 Poznan, Poland