The effect of hFVIII transgene on the chromosomal aneuploidy rate in rabbits

• Authors: J. Curlej , V. Parkanyi , J. Bulla , R. Jurcik , P. Chrenek
• Languages of publication: EN

Abstracts

EN

The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 mug/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.

Keywords

EN

BONE MARROW; CHROMOSOMAL ANEUPLOIDY; LYMPHOCYTE; RABBIT; TRANSGENE

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2007
• Volume: 55
• Issue: 3-4
• Pages: 161-164

Contributors

author

J. Curlej

author

V. Parkanyi

author

J. Bulla

author

R. Jurcik

author

P. Chrenek

• Document Type: SHORT REPORT
• Publication order reference: Peter Chrenek, Slovak Agricultural Research Centre in Nitra, Slovak Republic and Faculty of Natural Science, Constantine the Philosopher University, Nitra, Slovak Republic