PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2007 | 55 | 3-4 | 161-164
Article title

The effect of hFVIII transgene on the chromosomal aneuploidy rate in rabbits

Title variants
Languages of publication
EN
Abstracts
EN
The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 mug/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.
Publisher

Journal
Year
Volume
55
Issue
3-4
Pages
161-164
Physical description
Contributors
author
author
author
author
author
References
Document Type
SHORT REPORT
Publication order reference
Peter Chrenek, Slovak Agricultural Research Centre in Nitra, Slovak Republic and Faculty of Natural Science, Constantine the Philosopher University, Nitra, Slovak Republic
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-154f8991-9e76-3328-8f64-1e0696614574
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.